2013, Vol. 1, Issue 6, Part A

author(s)
Debmalya Das Gupta, Swati Sen Mandi

abstract
Zanthoxylum acanthopodium and Zanthoxylum oxyphyllum, found in North East India, are commercially importantrndue to its edible fruits and leaves containing essential oils used in cosmetics and perfume industries. These speciesrnhave also been used by local population as ethno medicine. We used amplified fragment length polymorphismrn(AFLP) to produce DNA fingerprints for two Zanthoxylum species viz. Z. acanthopodium, and. Z. oxyphyllum.rnTwelve collections (eight of Z. acanthopodium, four of Z. oxyphyllum) were used in the study. Six selective primerrnpairs were found to detect polymorphism. A total of 483 alleles were produced. Species-specific markers werernidentified in the two Zanthoxylum species (23 for Z .acanthopodium. and 13 for Z. oxyphyllum). The dendogramrnshowed species specific clustering of two species. The AFLP markers developed in this study could be used tornauthenticate two Zanthoxylum species to resolve adulteration-related problems faced by pharmaceutical industries tornsupplement conventional drug assessment protocols.