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Journal of Medicinal Plants Studies
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P-ISSN: 2394-0530, E-ISSN: 2320-3862

2015, Vol. 3, Issue 6, Part A

HPLC-Analysis of narcissin in flowers of Calendula officinalis

Kurkin Vladimir A, Kurkina Anna V, Afanasyeva Polina V, Platonov Igor A, Pavlova Larisa V

With the using of HPLC the method of the quantitative determination of the dominant flavonoid, narcissin (3-O-rutinoside of isorhamnetin) in the flowers of Calendula officinalis L. there was developed. It was determined that the content of narcissin in the in the flowers of this plant varies from 0,68±0,02% to 0,97±0,03%. The developed method of HPLC-analysis can also be used to determine the authenticity of the flowers of Calendula officinalis by means of the detection of narcissin, which has the diagnostic significance.
HPLC chromatogram of the alcoholic solution of narcissin (1).
Fig.: HPLC chromatogram of the alcoholic solution of narcissin (1).
Pages : 16-18 | 3963 Views | 155 Downloads
How to cite this article:
Kurkin Vladimir A, Kurkina Anna V, Afanasyeva Polina V, Platonov Igor A, Pavlova Larisa V. HPLC-Analysis of narcissin in flowers of Calendula officinalis. J Med Plants Stud 2015;3(6):16-18.
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