However, the traditional or conventional and old micro-propagation technique cannot be applicable in increasing and maintaining of demand of this herb which is used as very important raw substance for the preparation of therapeutic and pharmaceutical products in medicinal industries because of these reason the application and cultivation of in vitro micro-propagation methods of shwagandha plants can be the another most effective method for the regular and continuous possibility of maintaining demand and supply or distribution of several plantlet which is in stock of Withania for large scale field mass cultivation i.e. mass propagation in vitro culture room. In vitro shoot differentiation and micro-propagation of Withania somnifera from various different small excised explants such as hypocotyl and cotyledonary leaf, shoot tip, node,rninternode. Optimal normal growth, multiplication and development of Withania somnifera through morphogenesis in vitro processes of many tissues may vary for several different plants based on important nutritional requirements of plants. Basic nutrient basal media that are most frequently applicable and used include MS (Murashige and Skoog) medium, Gamborg (B5) medium, Linsmaier and Skoog (LS) medium and Nitsch and Nitsch (NN) medium.
Naveen Gaurav, AP Singh, Arun Kumar, Deepak Som, Deepak Kumar, Komal, Hira Singh Gariya. In vitro tissue culture studies from nodal and petiole explants of wild and cultivated traits of Withania somnifera in MS and B5 medium. Journal of Medicinal Plants Studies. 2016; 4(2): 92-96.