2016, Vol. 4, Issue 4, Part B

author(s)
Huerta Aguilar Ivone, Molina Torres Jorge, Garnica Romo Ma. Guadalupe, Yahuaca Juárez Berenice

abstract
Background: Fungi of the Ganoderma genus contain bioactive components such as terpenoids, polysaccharides, steroids, phenolic compounds and glycoproteins. Polysaccharides, triterpenes and phenolic compounds have antioxidant properties. Objective: To determine the antioxidant activity of the hydro alcoholic and the ethanolic extracts, and polysaccharides content of Ganoderma curtisii collected in Michoacan, Mexico. Methods: Hydroalcoholic and ethanolic extracts were obtained by direct heating at 78 °C and 80 °C respectively. Polysaccharides were extracted with water at 85 °C and then ethanol precipitation. The percentage inhibition of the ethanolic extract, hydroalcoholic extract and polysaccharides was evaluated by the DPPH method using ascorbic acid as a reference standard. Total polyphenol content was determined by the Folin-Ciocalteu method using gallic acid as standard. Results: Scavenging effects from Ganoderma extracts on DPPH radical increased with the concentrations of the extract. The maximum inhibition percentage for ethanol extracts, hydroalcoholic extracts and polysaccharides at a concentration of 1mg/ml, was 90.5%, 89.10% and 83.09%, respectively, while that of ascorbic acid was 96.4%. Phenolic compounds present in the hydroalcoholic extracts (35.6313 ± 0.1868 mg GAE/g) and ethanol (49.1467±0.1692 mg GAE/g) also contribute to increased antioxidant activity observed in these extracts. Conclusions: These results suggest that analyzed fungi are of potential interest as sources of strong natural antioxidants that could be used in the food nutrition and industries.