HPLC analysis of Ethynylestradiol from seed extract of Moringa oleifera Lam
Asha Kadam, BM Gaykar and Dr. RJ Barnabas
Moringa oleifeira Lam is common Indian plant belongs to monogeneric Moringaceae. The research investigated the presence etynyl etradiol in ‘Drumstick’ (Moringa oleifera Lam.) seed samples. The major compounds found in Moringa oleifera Lam. seeds were further analyzed by HPLC technique. High pressure liquid chromatography (HPLC) has been found to be an efficient and sensitive method for identification and quantification of different classes of Ethynylestradiol. HPLC studies showed that analysis of Ethynylestradiol derivatives extracted in petroleum ether and aqueous seed extracts of Moringa oleifera Lam. revealed significantly high levels. The calculated value of ethynyl estradiol was obtained 108.199 µg/ml of petroleum extract while it was obtained 655.26 µg/ml of Moringa oleifera aqueous extract. As the concentration of the samples increased, the peak area was also increased.