2018, Vol. 6, Issue 6, Part C

author(s)
Ferdinand M Adounkpe, Thierry CM Medehouenou, John R Klotoe and Victorien T Dougnon

abstract
The phytochemical screening of Crateva religiosa G. Forst, a plant used in Benin in traditional veterinary medicine, has shown its richness in alkaloids. The objective of this study was to evaluate the antibacterial pharmacochemical activity "in vitro" of total alkaloids extracts of C. religiosa leaves and roots on pathogenic germs in comparison to Amoxicillin + clavulanic acid (AMC), a conventional broad-spectrum antibiotic. The extraction of these alkaloids was made by the Stas-Otto method followed by thin layer chromatography. Total alkaloid extracts from leaves and roots were found to be more active than AMC against species of Staphylococcus aureus (38), Escherichia coli (28), Klebsiella pneumoniae (26), Streptococcus agalactiae (23), and Citrobacter freundii (12)following agar-well diffusion method using two concentrations (50 mg/ml and 200 mg/ml). Total alkaloids extracts of C. religiosa leaves and roots (200 mg/ml) gave the largest inhibition diameters against all microorganisms tested compared to AMC. The minimum inhibitory concentration was estimated at 50 mg/ml. The results obtained confirmed the long-established antimicrobial activity of this plant and brought novelty that its alkaloids would intervene in its therapeutic effect.