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Journal of Medicinal Plants Studies
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ISSN Print: 2394-0530, ISSN Online: 2320-3862, NAAS Rating: 3.53

NAAS Rating: 3.53 NEW

Journal of Medicinal Plants Studies

2013, Vol. 1, Issue 3, Part B
Antioxidant and Antimicrobial Activity of Pleurotus Florida Cultivated in Bangladesh

Mohammad Mijanur Rahman, Asiqur Rahaman, Taslima Nahar, Borhan Uddin, Mafroz Ahmed Basunia, Shahdat Hossain*

Mushroom, a new food item in Bangladesh, has remained largely obscure about its nutritional and medicinal values.rnEvaluation of antioxidant and antimicrobial activity of the Pleurotus florida ethanol extract was the purpose of thernpresent study. Crude P. florida extract was qualitatively screened for the presence of phytochemicals while totalrnpolyphenol content was measured quantitatively. DPPH-free radical scavenging activity, total reducing power andrnability of inhibiting in vitro Fenton’s reagent-induced oxidative stress were used to evaluate the antioxidantrnpotentials of the P. florida extract. Minimum inhibitory concentration and antibiogram of the P. florida extract wasrnassessed against six human pathogenic bacteria. Ethanol extract of P. florida possesses appreciable antioxidantrnactivity, as indicated by the polyphenol contents, DPPH scavenging activity, reducing power and anti-lipid peroxiderneffect. The extract exhibited antimicrobial activity against all of the microorganisms tested.
DPPH-scavenging activity of P. florida . (A). The IC50 values of P. florida . Data are the mean ± SEM of triplicate determinations. IC50 values were calculated by fitting the data to nonlinear regression analysis equitation [dose-response]. (B). Comperative DPPH- deactivating activity of BHT and P. florida . TLC plate was stained initially with 8.0 l solution DPPH. After air dry the spots were reloaded with 8 µl volume of butylated hydroxyl toluene (BHT) and P. florida extract at equimolar concentration. The stable DPPH free radicals were clearly deactivated by the antioxdants (BHT and extract), as indicated by the gradual lightening of the DPPH’s purple color of the spots. The scavenging effects were dose dependent. The color of the spots was digitized and calculated by using NIH ImageJ analyzer (Right panel).
Fig.: DPPH-scavenging activity of P. florida . (A). The IC50 values of P. florida . Data are the mean ± SEM of triplicate determinations. IC50 values were calculated by fitting the data to nonlinear regression analysis equitation [dose-response]. (B). Comperative DPPH- deactivating activity of BHT and P. florida . TLC plate was stained initially with 8.0 l solution DPPH. After air dry the spots were reloaded with 8 µl volume of butylated hydroxyl toluene (BHT) and P. florida extract at equimolar concentration. The stable DPPH free radicals were clearly deactivated by the antioxdants (BHT and extract), as indicated by the gradual lightening of the DPPH’s purple color of the spots. The scavenging effects were dose dependent. The color of the spots was digitized and calculated by using NIH ImageJ analyzer (Right panel).
Pages : 166-175 | 1094 Views | 24 Downloads
How to cite this article:
Mohammad Mijanur Rahman, Asiqur Rahaman, Taslima Nahar, Borhan Uddin, Mafroz Ahmed Basunia, Shahdat Hossain*. Antioxidant and Antimicrobial Activity of Pleurotus Florida Cultivated in Bangladesh. J Med Plants Stud 2013;1(3):166-175.
Journal of Medicinal Plants Studies
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