DPPH-scavenging activity of P. florida . (A). The IC50 values of P. florida . Data are the mean ± SEM of triplicate determinations. IC50 values were calculated by fitting the data to nonlinear regression analysis equitation [dose-response]. (B). Comperative DPPH- deactivating activity of BHT and P. florida . TLC plate was stained initially with 8.0 l solution DPPH. After air dry the spots were reloaded with 8 µl volume of butylated hydroxyl toluene (BHT) and P. florida extract at equimolar concentration. The stable DPPH free radicals were clearly deactivated by the antioxdants (BHT and extract), as indicated by the gradual lightening of the DPPH’s purple color of the spots. The scavenging effects were dose dependent. The color of the spots was digitized and calculated by using NIH ImageJ analyzer (Right panel).
How to cite this article:
Mohammad Mijanur Rahman, Asiqur Rahaman, Taslima Nahar, Borhan Uddin, Mafroz Ahmed Basunia, Shahdat Hossain*. Antioxidant and Antimicrobial Activity of Pleurotus Florida Cultivated in Bangladesh. J Med Plants Stud 2013;1(3):166-175.