DPPH-scavenging activity of P. florida . (A). The IC50 values of P. florida . Data are the mean ± SEM of triplicate determinations. IC50 values were calculated by fitting the data to nonlinear regression analysis equitation [dose-response]. (B). Comperative DPPH- deactivating activity of BHT and P. florida . TLC plate was stained initially with 8.0 l solution DPPH. After air dry the spots were reloaded with 8 µl volume of butylated hydroxyl toluene (BHT) and P. florida extract at equimolar concentration. The stable DPPH free radicals were clearly deactivated by the antioxdants (BHT and extract), as indicated by the gradual lightening of the DPPH’s purple color of the spots. The scavenging effects were dose dependent. The color of the spots was digitized and calculated by using NIH ImageJ analyzer (Right panel).
The effect of P. florida on in vitro oxidative stress in the brain tissues. The bars represent the mean ± SEM (n=3). Con = Control; Os = Fenton’s reagent-induced oxidative stress; Os + V-E = Vitamin E plus Fenton’s reagentinduced oxidative stress; Os+ P.f.= P. florida extract plus Fenton’s reagent-induced oxidative stress. Data were analyzed by One-way ANOVA followed by Tukey’s least square differences test for post hoc comparisons. Bars with different alphabets are significantly different at P<0.05 level.