2013, Vol. 1, Issue 3, Part B

author(s)
Vivek Sharma

abstract
In the present study, a simple, sensitive, selective and reliable HPLC method based on photo diode array detectorrn(PDA) has been developed and validated for the simultaneous determination of an important compoundrn(podophyllotoxin) in leaves and rhizomes parts of four different populations of Podophyllum hexandrum Royle fromrnNorth Indian Himachal Pradesh higher altitude Himalayas. The analysis was carried out on a RP-18e (LiCrosphere,rn5µm, 250 x 4.0 mm) column, with isocratic elution of acetonitrile: water (40:60, v/v). The method was validated forrnaccuracy, precision, limit of detection and quantification. The regression equation revealed a good linear relationshiprn(r2 = 0.9999 for podophyllotoxin) within test ranges. The limit of detection and limit of quantification for analyte inrnPDA was 50 and 110µg, respectively. The method showed good reproducibility for the quantification ofrnpodophyllotoxin in P. hexandrum