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Journal of Medicinal Plants Studies
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ISSN Print: 2394-0530, ISSN Online: 2320-3862, NAAS Rating: 3.53

NAAS Rating: 3.53 NEW

Journal of Medicinal Plants Studies

2013, Vol. 1, Issue 3, Part B
Part Based HPLC-PDA Quantification of Podophyllotoxin in Populations of Podophyllum hexandrum Royle “Indian Mayapple” from Higher Altitude Himalayas

Vivek Sharma

In the present study, a simple, sensitive, selective and reliable HPLC method based on photo diode array detectorrn(PDA) has been developed and validated for the simultaneous determination of an important compoundrn(podophyllotoxin) in leaves and rhizomes parts of four different populations of Podophyllum hexandrum Royle fromrnNorth Indian Himachal Pradesh higher altitude Himalayas. The analysis was carried out on a RP-18e (LiCrosphere,rn5µm, 250 x 4.0 mm) column, with isocratic elution of acetonitrile: water (40:60, v/v). The method was validated forrnaccuracy, precision, limit of detection and quantification. The regression equation revealed a good linear relationshiprn(r2 = 0.9999 for podophyllotoxin) within test ranges. The limit of detection and limit of quantification for analyte inrnPDA was 50 and 110µg, respectively. The method showed good reproducibility for the quantification ofrnpodophyllotoxin in P. hexandrum
HPLC chromatogram of a leaf sample PHL-1 (3300m) showing peak of podophyllotoxin.
Fig.: HPLC chromatogram of a leaf sample PHL-1 (3300m) showing peak of podophyllotoxin.
Pages : 176-183 | 1051 Views | 19 Downloads
How to cite this article:
Vivek Sharma. Part Based HPLC-PDA Quantification of Podophyllotoxin in Populations of Podophyllum hexandrum Royle “Indian Mayapple” from Higher Altitude Himalayas. J Med Plants Stud 2013;1(3):176-183.
Journal of Medicinal Plants Studies
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