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Journal of Medicinal Plants Studies
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ISSN Print: 2394-0530, ISSN Online: 2320-3862, NAAS Rating: 3.53

NAAS Rating: 3.53 NEW

Journal of Medicinal Plants Studies

2014, Vol. 2, Issue 2, Part A
Phytochemical investigation on leaf extract of Adhatoda schimperiana, Ethiopia

Habtamu Abebe*, Belayhun Kibret, Adane Haile

Interest in obtaining biologically active compounds from natural sources has recently spiked due to their low toxicity, complete biodegradability, availability from renewable sources, and in most cases, low cost. This research project is aimed at isolating, phytochemical screening and partial characterizing the chemical constituents of Adhatoda schimperiana of Ethiopia. The leaves of Adhatoda schimperiana was extracted with 1.5 L of n-Hexane for 72 h. The extract was evaporated under reduced pressure at temperature of 40 °C using rotary evaporator. Partitioning was done using n-hexane, chloroform, ethyl acetate, ethanol and methanol. The organic and aqueous layer was concentrated to dryness under reduced pressure to afforded 3.10 g and 2.50 g crude extract respectively. Phytochemical screening revealed the presence of alkaloids, polyphenols, flavonoids, glycosides, phytosterols, saponins, triterpenes, and quinines as a major class of compounds. However, less presence of tannins was observed. The selective extraction of triterpenes allowed me to obtain 0.71% yields. Separation on column chromatography conducted to a major fraction of triterpenes and a major compound from defatted pricarp leaves of chloroform extract.Partial characterization of the pure compounds was done using a combination of spectroscopic techniques including UV-Vis, IR, 1D NMR (1H-NMR,13C-NMR and DEPT).
Dept Spectera of compound ASH-14
Fig.: Dept Spectera of compound ASH-14
Pages : 23-31 | 1203 Views | 55 Downloads
How to cite this article:
Habtamu Abebe*, Belayhun Kibret, Adane Haile. Phytochemical investigation on leaf extract of Adhatoda schimperiana, Ethiopia. J Med Plants Stud 2014;2(2):23-31.
Journal of Medicinal Plants Studies
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