The assessment of the effect of extracts of Bauhinia variegata against the compound 48/80-induced mast cell degranulation and systemic anaphylaxis
Khursheed Ahmad Ansari
Aim: The present investigation was designed to evaluate the possible effect of extracts of B. variegata against the compound 48/80-induced mast cell degranulation and systemic anaphylaxis.
Methods: The authenticated plant material was defatted using petroleum ether and extracted with ethanol and distilled water to obtain ethanol (BVE) and aqueous (BVA) extract, respectively. The stabilization potential was studied on mouse mast cells and anti-anaphylactic activity was evaluated by determining the mortality rate of mice treated with toxicant (compound 48/80).
Results: The HPLC analysis recorded 2.09% of quercetin and 0.755% gallic acid in BVE and 0.07% and 8.16% in BVA, respectively. In acute toxicity study, no mortality and behavioral changes were observed up to a dose of 2,000 mg.kg-1 p.o. In the compound 48/80- induced mast cell degranulation study, both BVE and BVA showed protection of mast cell degranulation in a dose-dependant manner. The table showed slightly better inhibition revealed by BVE (71.18%) at a dose level of 400 mg.kg-1 when compared with standard drug DSCG (70.95%). The lower rate of mortality of mice from compound 48/80- induced anaphylactic shock was 50% at 400 mg.kg-1 (BVE) whereas BVA had shown lesser effect on the anaphylactic shock.
Conclusion: It is noteworthy that the extent and potency of inhibition shown by BEagainst compound 48/80-induced mast cell degranulation and anaphylaxiswas almost similar to that of DSCG. The results of the study demonstratedthat the ethanol extract of B. variegata showed marked mast cell stabilizationand anti-anaphylactic activities.
Khursheed Ahmad Ansari. The assessment of the effect of extracts of Bauhinia variegata against the compound 48/80-induced mast cell degranulation and systemic anaphylaxis. J Med Plants Stud 2014;2(4):73-76.