Contact: +91-9711224068
Journal of Medicinal Plants Studies
  • Printed Journal
  • Indexed Journal
  • Refereed Journal
  • Peer Reviewed Journal

ISSN Print: 2394-0530, ISSN Online: 2320-3862, NAAS Rating: 3.53

NAAS Rating: 3.53 NEW

Journal of Medicinal Plants Studies

2015, Vol. 3, Issue 3, Part A
Optimization of in vitro culture conditions for accumulation of diosgenin by fenugreek

Joanna Ciura, Magdalena Szeliga, Mirosław Tyrka

Fenugreek as a spice, vegetable and medicinal plant has valuable nutritional properties and is important for diversification of human diet. The most important medicinal properties of this herb are related with the content of diosgenin. Selection of media for optimal and stable production of diosgenin level in plants grown in vitro is prerequisite for understanding the biochemical pathway of diosgenin biosynthesis. Changes of concentration of this secondary metabolite in various plant organs during growth are also critical for sampling and isolation procedure. We found that, the concentration of diosgenin depends on the day of harvest, type of medium and plant organ. The highest content of diosgenin in leaves occurs between 21st and 38th day of growth. These results suggest a positive influence of carbohydrates on the efficiency of diosgenin synthesis. The highest content of diosgenin was produced by leaves when compared to stems, roots and callus culture.
Content of diosgenin in fresh (left) and dry (right) weight of various parts of fenugreek plant and calli. Squares denote standard error, and dashed lines represent 95% confidence intervals.
Fig.: Content of diosgenin in fresh (left) and dry (right) weight of various parts of fenugreek plant and calli. Squares denote standard error, and dashed lines represent 95% confidence intervals.
Pages : 22-25 | 1094 Views | 21 Downloads
How to cite this article:
Joanna Ciura, Magdalena Szeliga, Mirosław Tyrka. Optimization of in vitro culture conditions for accumulation of diosgenin by fenugreek. J Med Plants Stud 2015;3(3):22-25.
Journal of Medicinal Plants Studies
Please use another browser.