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Journal of Medicinal Plants Studies
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ISSN Print: 2394-0530, ISSN Online: 2320-3862, NAAS Rating: 3.53

NAAS Rating: 3.53 NEW

Journal of Medicinal Plants Studies

2015, Vol. 3, Issue 4, Part A
Protocol Establishment for Multiplication and Regeneration of ‘Holy Basil’ (Ocimum sanctum Linn). An Important Medicinal plant with High Religious Value in India
Regenerated plants of O. sanctum maintained in green house on a potting mixture of 2:1 of compost and soil.
Fig.: Regenerated plants of O. sanctum maintained in green house on a potting mixture of 2:1 of compost and soil.
Root induction from in vitro regenerated shoots of O. sanctum on half strength MS medium supplemented with IBA (1.5 mg/l) after five weeks of transfer.
Fig.: Root induction from in vitro regenerated shoots of O. sanctum on half strength MS medium supplemented with IBA (1.5 mg/l) after five weeks of transfer.
Shoot elongation and multiplication of O. sanctum on MS medium supplemented with BA (1.0 mg/l) and IAA (0.5 mg/l) after first subculture
Fig.: Shoot elongation and multiplication of O. sanctum on MS medium supplemented with BA (1.0 mg/l) and IAA (0.5 mg/l) after first subculture
Shoot induction and bud break response of O. sanctum on MS medium supplemented with BA (0.5 mg/l) and IAA (0.5 mg/l) after two weeks of culture.
Fig.: Shoot induction and bud break response of O. sanctum on MS medium supplemented with BA (0.5 mg/l) and IAA (0.5 mg/l) after two weeks of culture.
Callus induction from the leaf explants of O. sanctum after 4 weeks of culture in MS medium supplemented with different concentrations of picloram. (a) 0 mg/L; (b) 1 mg/L; (c) 3 mg/L; (d) 5 mg/LShoot induction and bud break response of O. sanctum on MS medium supplemented with BA (0.5 mg/l) and IAA (0.5 mg/l) after two weeks of culture.
Fig.: Callus induction from the leaf explants of O. sanctum after 4 weeks of culture in MS medium supplemented with different concentrations of picloram. (a) 0 mg/L; (b) 1 mg/L; (c) 3 mg/L; (d) 5 mg/LShoot induction and bud break response of O. sanctum on MS medium supplemented with BA (0.5 mg/l) and IAA (0.5 mg/l) after two weeks of culture.
Complete plant Ocimum sanctum
Fig.: Complete plant Ocimum sanctum
Journal of Medicinal Plants Studies
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