2015, Vol. 3, Issue 4, Part A

Fig.: Regenerated plants of O. sanctum maintained in green house on a potting mixture of 2:1 of compost and soil.

Fig.: Root induction from in vitro regenerated shoots of O. sanctum on half strength MS medium supplemented with IBA (1.5 mg/l) after five weeks of transfer.

Fig.: Shoot elongation and multiplication of O. sanctum on MS medium supplemented with BA (1.0 mg/l) and IAA (0.5 mg/l) after first subculture

Fig.: Shoot induction and bud break response of O. sanctum on MS medium supplemented with BA (0.5 mg/l) and IAA (0.5 mg/l) after two weeks of culture.

Fig.: Callus induction from the leaf explants of O. sanctum after 4 weeks of culture in MS medium supplemented with different concentrations of picloram. (a) 0 mg/L; (b) 1 mg/L; (c) 3 mg/L; (d) 5 mg/LShoot induction and bud break response of O. sanctum on MS medium supplemented with BA (0.5 mg/l) and IAA (0.5 mg/l) after two weeks of culture.

Fig.: Complete plant Ocimum sanctum