2015, Vol. 3, Issue 5, Part A

author(s)
S Kirubanandan, Bharathi Ravi, S Renganathan

abstract
The controlling and eradication of wound infection is a challenging aspect for the management of open wounds. Despite the use of synthetic antimicrobial agents, drug resistance and cytotoxicity hinder the activity of those antimicrobial agents and thereby increase the chances of infection and delay regeneration of epidermis and dermis at the wound site. Additionally, the microbial enzymes secreted by wound pathogens degrade proteins of extracellular matrix at the wound site. As a consequence, The ECM proteins was degraded and delayed wound closure. The present study investigates the influence of Triphala on antimicrobial activity and enzyme inhibition activity which will be used in wound healing studies. The methanolic extract of triphala was prepared and its antimicrobial activity was tested against Pseudomonas aeruginosa. The activity of triphala extract against metalloprotease was studied by Zymography. Enzymatic activities were detected as clear bands of gelatin lysis against a dark background. The 18±2mm clear zone in disc diffusion assay and minimal inhibitory concentration (MIC) of 7.8125mg/ml against pseudomonas control strains clearly confirmed the antibacterial activity of Triphala. Zymography analysis exhibited greater reduction in protease activity at ≥1500μg/ml. By virtue of the inhibitory effect of Triphala on Pseudomonas aeruginosa and its enzymes such as proteases, it could be potentially used as a new therapeutic agent for pseudomonas infected dermal wounds. The results hence highlighted the beneficial effects of the topical application of Triphala would be used in the acceleration of wound healing and its effect on controlling wound infections at wound site.