2015, Vol. 3, Issue 5, Part C

author(s)
Missa Mohammed Saleh Abde Alsiede, Malik A Abddrahman, Ahmed E M Saeed

abstract
The leaves and seeds of Cassia Singueana (Leguminosae), one of the most popular herbal products in tropical countries, are used in traditional medicine for treatment of several diseases and ailments. The present study was conducted to investigate the anti-oxidative activities of different solvent extracts of Cassia Singueana leaves and seeds. The results indicate that all the extracts have reducing power DPPH radical scavenging abilities. Ethyl acetate and methanol extract of the leaves extracts have the highest total reducing power whereas, the methanol and ethyl acetate extracts of the seeds part have more potent free radical scavenging activity than all the other extracts using DPPH free radical scavenge capacity assay. IC50 was calculated and compared with propyl gallate as standard. Qualitative Phytochemical screening of the two parts indicates the presence of alkaloids, coumarins, flavonoids, sterols, saponins and tannins with different concentration. Quantitative analysis of the two parts of Cassia Singueana for phenolic flavonoids and tannins compounds revealed that the total phenolic content ranged from 122.75 to 376.1 and 286.94 to 1990 mg/g of dry weight of leaves and seeds extracts respectively which expressed as gallic acid equivalents. The total flavonoid concentrations varied from 42 to 1020 mg/g and 188.34 to 449 mg/g of dry weight of leaves and seeds extracts respectively that expressed as Qurestin equivalents. The total tannins concentrations varied from 126.21 to 1323 and 309.88 to 1710 mg/g for leaves and seeds respectively. It could be conducted that leaves and seeds parts of C. Singueana Possessed anti-oxidative activities and can be used as a potential alternative medicine for oxidative stress related to non-communicable chronic diseases. Further experimental and clinical studies including laboratory animals are warranted.