Monitoring antioxidant and antityrosinase activity of clove aromatic flower buds
The aim of the study is to investigate the In vitro antioxidant activity, total phenol and flavonoid content of clove bud (syzygium aromaticum) and compared with that of oil extracted from clove bud oil. Antioxidant potential was evaluated using DPPH radical scavenging and Hydrogen peroxide scavenging assays. The total phenolic content was measured by using folin-Ciocalteau method while total total flavonoid content was determined using AlCl3 calorimetric method in both clove bud extracts and clove bud oil. The anti-melanogenic effect of extracts of clove bud and clove bud oil was also examined by using Tyrosinase as a substrate. Clove bud oil showed (87.2+0.83%) DPPH radical scavenging as compared to methanolic extract of clove bud which was (82.9+3.1%) at 200 µg/ml concentration. However, under the same condition, the standard Ascorbic acid showed (91.4+3.4%) inhibition at same concentration. In addition, clove bud oil and clove bud had an effective H2O2 Scavenging activity and was compared with the potential of synthetic antioxidant BHT. The present study showed that clove bud and clove bud oil contains many healthnutrients and phytochemicals which are very useful for human health. Due to the presence of total phytochemicals such as phenols and flavonoids, the clove bud showed efficient antioxidant activity. The clove bud oil is much effective due to its capability to reduce free radicals as compared to clove bud.