2016, Vol. 4, Issue 3, Part A

author(s)
Muhammad Moin Uddin Mazumdar, Md. Ariful Islam, Mohammad Tanvir Hosen, Humaira Gulzar, Mohammad Abu Sayem Shakil, Mohammad Nazmul Alam, Md. Mominur Rahman, Dr. Shaikh Bokhtear Uddin

abstract
For the evaluation of potential anti-arthritic, thrombolytic and cytotoxic activities of methanolic and ethanolic leaf extract of Macaranga denticulata, bovine serum albumin induced anti-arthritic method, clot lysis method and brine shrimp lethality bioassay was performed. In the case of protein denaturation, methanolic and ethanolic leaf extract of M. denticulata showed 68.40±0.92%* and 63.35±1.22%* whereas standard diclofenac sodium exhibited 90.58±0.82% at 1000 µg/ml (highest conc.) respectively. At lowest concentration (31.25 µg/ml), methanolic and ethanolic leaf extract demonstrated 26.31±0.31%* and 22.44±0.42%* whereas diclofenac sodium showed 51.75±0.92 respectively. Both methanolic and ethanolic leaf extract exhibited promising thrombolytic activity which were 38.23±0.88*% and 33.52±1.64**% respectively whereas standard streptokinase showed 63.54±2.61%**. Methanolic and ethanolic leaf extract of this plant showed significant cytotoxic activity. LC₅₀ value of methanolic leaf extract was 74.30 µg/ml and ethanolic leaf extract was 97.77 µg/ml whereas 12.59 µg/ml cytotoxic effect was observed for the standard vincristine sulphate.