2016, Vol. 4, Issue 3, Part C

author(s)
Awantika Singh, Vikas Bajpai, Sunil Kumar, KB Ramesh kumar and Brijesh Kumar

abstract
Coscinium fenestratum is extensively used as medicinal plant in traditional medicine to treat variety of ailments. Protoberberine and aporphine alkaloids are reported the major components in stem and root of this plant. In present study, quantification of eight bioactive alkaloids in different plant parts of C. fenestratum was done using ultra-performance liquid chromatography coupled to hybrid triple quadrupole/linear ion trap mass spectrometry in multiple reactions monitoring acquisition. The analytical procedure was validated; obtaining precision and recoveries of the samples ranging from 0.40-3.1% and 0.59-3.17% (98-101%), respectively (expressed as relative standard deviations, RSDs). Calibration curves for all the eight analytes provided optimum linear detector response (with R² ³0.9988) over the concentration range of 0.5-1000 ng/mL. Finally, the method was applied to different plant parts of C. fenestratum, and observed that root part had higher level of total content whereas flower part had the lowest level of total content of eight analytes.