Antimicrobial activity of the essential oils of Lavandula stoechas flowers extracted by microwave
Sanaz Sadani and Alireza Shakeri
Lavandula stoechas genus is an important member of family Lamiaceae, are widely distributed in Golestan province of IRAN. Lavandula has been used in traditional medicine to treat various diseases. The chemical compounds of essence were identified by GC-MS analysis. In order to evaluate the antimicrobial effect of essential oil, Broth microdilution (MIC determine the minimum inhibitory concentration) methods was used. The antimicrobial activity was tested against on Gram Positive and Negative Bacteria (staphylococcus aureus, bacillus cereus, enterococcus faecalis, salmonella enteritidis, escherichia coli and pseudomonas aeruginosa). The yield of essential oil by microwave under optimum condition was obtained 0.112%. 28 components with 98.93% yields have been identified. The main compounds of essential oil obtained camphor, 1,8-Cineole, Linalool and borneol. The essential oil of L. stoechas p. showed antibacterial activity against most of the tested standard bacteria strains expects pseudomonas aeruginosa and enterococcus faecalis. Staphylococcus aureus and bacillus cereus with 29 and 32 mm respectively diameter was showed the highest sensitivity. MIC and MBC methods also show that all bacteria have the same minimum inhibitory and fatality concentrations except Enterococcus facials with minimum inhibitory concentration of 1.32 and minimum concentration fatality of 1.16. The results showed that the essential oil of Lavandula stoechas was revealed highly inhibitory antimicrobial activity especially on gram positive bacteria and can be used instead of chemical drugs to treat bacterial infections.