Estimation of alkaloid, saponin and flavonoid, content in various extracts of Crocus sativa
M Amin Mir, Kajal Parihar, Uzma Tabasum and Ekata Kumari
Crocus sativa is a well-known spice grown mostly in the Kashmir region of India. The flowers of the plant have a good aroma and are full of secondary metabolites due to which the flowers are being used dying agents and the flavoring agents in almost all types of dishes. The flowers of the concerned plant have been analysed for the estimation of Alkaloid, Saponin and Flavonoid contents. Three solvents viz DCM, Methanol and Water have been used for the extraction of Alkaloid, Saponin and Flavonoid contents. Different solvent extracts extract different concentration of metabolites. The alkaloid content was found to be (6.4 and 2.4mg/g) in the methanol and water extract of the flowers, similarly the flavonoid content was found to be 1.8, 9.2 and 11.2mg/g in DCM, Methanol and water extract respectively. The Saponin content was found to be 1.2 and 3.4mg/g in the methanol and water extract of the crocus sativa.