Phytochemical analysis and HPLC study of vitamin-C from Punica granatum L. Aarakta variety of India
Pavan C Akkiraju, Dipeeka D Suryawanshi, Aparna J Jawakekar, Harshad S Tambe and Srilakshmi Mamillapalli
Punica granatum L. commonly known as Pomegranate is a plant with medicinal properties known from ancient times. The Indian variety Aarakta was investigated for its phytochemicals along with HPLC study of Vitamin-C. Methanol extracts of exocarp, mesocarp and seeds were evaluated for alkaloids, flavonoids, tannins, anthraquinones, glycosides, phycobilins, vitamin-C, amino acids, proteins, carbohydrates and saponins. All three parts of the fruit contained saponins, carbohydrates, alkaloids, phycobilins and vitamin-C. The same were found absent with glycosides, proteins, amino acids and anthraquinones. The flavonoids were not found in the seeds and gallotannins were present only in the seeds. HPLC analysis of Vitamin-C had TP contents ranged from 53.7 to 5917.3 μg/mg, 4.0 to 3957.3μg/mg and 497.4 to 2797.7μg/mg for exocarp, mesocarp and seed extracts, respectively. The contents of TF ranged from 0.65 to 1.6 μg/mg, 0.5 to 1.9 μg/mg and 0.60 to 1.61 μg/mg for exocarp, mesocarp and seed extracts, respectively.
Pavan C Akkiraju, Dipeeka D Suryawanshi, Aparna J Jawakekar, Harshad S Tambe, Srilakshmi Mamillapalli. Phytochemical analysis and HPLC study of vitamin-C from Punica granatum L. Aarakta variety of India. J Med Plants Stud 2016;4(6):09-12.