Blood sugar lowering action of modified herbal formulation based on common spices
Prabir Kr. Sinha Mahapatra, Tapas Kumar Sur, Alok K Hazra and Subhas C Mandal
The present study was designed to prepare two herbal formulations, PMM1 and PMM2 using five common Indian herbs. The preclinical efficacies of these formulations were studied in alloxan induced diabetic rats. The formulations (PMM1 and PMM2) were prepared with extracts of Tinospora cordifolia, Trigonella foenum-graccum, Scoparia dulcis, Adhatoda vasica and Cassia occidental. The formulations are standardized using phytochemical analyses and HPTLC chromatographic fingerprints. Anti-hyperglycaemic activities of test drugs were evaluated on alloxan induced (120 mg/kg, i.p) diabetic rats. PMM1 (50-150 mg/kg, p.o) exhibited best potentiality in reducing blood glucose within 14 days treatment in comparison with PMM2 at the same doses levels. PMM1 treatments also significantly (p<0.5) negated elevations of blood cholesterol, triglycerides, urea and creatinine in alloxan diabetic rats. The observed effects were also comparable with Glibenclamide (5 mg/kg). The preset observation identified formulation PMM1 for anti-hyperglycemic effect and substantiates its uses on diabetic population.