In vitro regeneration of shoots from apical and auxiliary bud explants of Coffea arabica L
Esayas Aga and Yashwant Khillare
The aims of the present investigation were to optimize tissue culture protocol for C. arabica, to identify the best explants and growth hormone combinations and their respective concentrations suitable for Coffea Arabica L. in vitro multiplication. In a present investigation, surface sterilized apical and auxiliary bud explants were cultured on MS media for shoot regeneration and multiplication. The media were prepared in four different combinations of auxins and cytokinins supplemented with 30 g/l sucrose, 4 g/l agar and 10 mg/l L-Cysteine HCl, L-Cysteine HCl was used to control the oxidation of phenolic compounds. A similar experiment was repeated under dark condition by covering the culture vessels with black polyethylene plastic bags to study the effect of darkness on growth response of the explants. Each treatment consists of 5 replicates in a completely randomized design. The results showed that both bud explants regenerated shoots in all the four media employed, and shoots regenerated from apical bud explants were vigorous than those regenerated from auxiliary bud explants. Results are discussed from C. arabica L. tissue culture protocol optimization point of view.