Determination of steroid compounds profile in three Polygonum species by HPTLC
B Ezhilan and R Neelamegam
HPTLC analysis was carried out to determine the steroid compounds profile in the whole-plant samples of selected Polygonum species (P. chinense, P. glabrum and P. barbatum). The methanol extract of whole-plant samples obtained from Polygonum species (P. chinense, P. glabrum and P. barbatum) showed 6, 6 and 5 compounds, respectively, and were compared with stigmasterol and solasodine standards. Among the compounds, 4, 3 and 2 compounds in each sample, respectively, was identified as steroids while the others were unknown. Two steroid compounds each from P. chinense and P. glabrum showing same peak Rf values (0.69/0.89, respectively). Similarly, one unknown compound each from P. glabrum and P. barbatum also showed same peak Rf values (0.14), while all other compounds of Polygonum species showed no similarities in their peak Rf values of compounds detected. The HPTLC analysis of whole plant methanol extracts of Polygonum species shows variations in the nature and number of steroids from one another.