2017, Vol. 5, Issue 1, Part B

author(s)
B Ezhilan and R Neelamegam

abstract
HPTLC analysis was carried out to determine the steroid compounds profile in the whole-plant samples of selected Polygonum species (P. chinense, P. glabrum and P. barbatum). The methanol extract of whole-plant samples obtained from Polygonum species (P. chinense, P. glabrum and P. barbatum) showed 6, 6 and 5 compounds, respectively, and were compared with stigmasterol and solasodine standards. Among the compounds, 4, 3 and 2 compounds in each sample, respectively, was identified as steroids while the others were unknown. Two steroid compounds each from P. chinense and P. glabrum showing same peak R_f values (0.69/0.89, respectively). Similarly, one unknown compound each from P. glabrum and P. barbatum also showed same peak R_f values (0.14), while all other compounds of Polygonum species showed no similarities in their peak R_f values of compounds detected. The HPTLC analysis of whole plant methanol extracts of Polygonum species shows variations in the nature and number of steroids from one another.