HPTLC analysis of coumarin profile in the leaf and bark samples of Loranthus longiflorus Desr. (Syn.–Dendrophthoe falcata (L.f.) Ettingsh) collected from two host trees
Chandrakasan L and Neelamegam R
Coumarin compounds profile was determined by HPTLC in the methanol extract of leaf and bark samples of Loranthus longiflorus collected from two host trees– Casuarina equisetifolia and Ficus religiosa and compared. The methanol extract of Loranthus leaf samples obtained from Casuarina host trees showed ten compounds while it was nine compounds in the leaf samples collected from Ficus host tree and were compared with coumarin standard. Among the compounds, 3 and 2 compounds in each sample, respectively was identified as coumarin while all other compounds were unknown. Two compounds from each leaf samples collected from Casuarina equisetifolia (peak no. 4 & 10) and Ficus religiosa (peak no. 1 & 9) host trees showed similar Rf values (0.22 & 0.94, respectively). On the other hand, the methanol extract of Loranthus longiflorus bark sample collected from Casuarina equisetifolia and Ficus religiosa host trees contained 9 and 4 compounds in each sample, respectively, and were compared with coumarin standard. All these compounds were unknown. Among the compounds, 3 compounds from both bark samples obtained from Casuarina equisetifolia (peak no. 1, 4, & 6) and Ficus religiosa (peak no. 1, 3, & 4) showed similar Rf values (0.04, 0.36 & 0.52). None of the compounds from leaf and park samples of Loranthus longiflorus collected from Casuarina equisetifolia and Ficus religiosa showed similar Rf values.
Chandrakasan L, Neelamegam R. HPTLC analysis of coumarin profile in the leaf and bark samples of Loranthus longiflorus Desr. (Syn.–Dendrophthoe falcata (L.f.) Ettingsh) collected from two host trees. J Med Plants Stud 2017;5(1):135-139.