2017, Vol. 5, Issue 1, Part E

The present study was conducted to investigate the phytochemical contents and evaluate the antimicrobial property of Sargassum oligocystum and Sargassum crassifolium extracts collected from the coast at Diora-Zinungan, Santa Ana, Cagayan, Philippines. Extracts of powdered seaweed were prepared using sequential extraction with different organic solvents in order to increasing the polarity (Ethanol, n-hexane, dichloromethane and ethyl acetate and aqueous). Five fractions (Ethanol, n-hexane, dichloromethane ethyl acetate and aqueous) were examined for antimicrobial activity by using disc diffusion assay on thirteen (13) strains of aquaculture pathogen. The extracts showed the presence of phytochemical constituents like flavonoids, tannins, phenolics, sterols and terpenoids and the absence of saponins. Among the tested extracts for antimicrobial activity, ethanolic extracts was determined to be the best solvent for isolation of antimicrobial compounds from the tested seaweeds followed by n-hexane, dichloromethane ethyl acetate and aqueous. The extract of the S. oligocystum and S. crassifolium showed presence of different groups of secondary metabolites such as flavonoids, tannins, phenolics, sterols and terpenoids which are important indicators of the microbial properties of seaweeds.