Design and development of polyherbal formulation with antioxidant potential
DP Shelke, GR Shendarkar, AK Daswad, JG Pohare, SN Wangawar and AB Roge
Ayurvedic system of medicine is as old as human civilization. Present study involves the development of a polyherbal formulation by using four different herbs i.e. Pumpkin seed (Cucurbita maxima), Sunflower seed (Helianthus annus), Spinach leaves (Spinach oleracea), and Beet root (Beta vulgaris). Ethanol (90%) was used for preparation of polyherbal tincture. Freshly collected and authenticated herbs were characterized by studying its morphological and pharmacognostic character. Further the powdered drugs and its tincture was subjected to its physicochemical evaluation. Phytochemical screening showed the presence of alkaloids, glycosides, carbohydrates, amino acid, tannin, steroids and flavonoids in the tincture. Physical parameters like solubility, pH, ash values, LOD, extractive value etc. has been studied. The antioxidant activity of the tincture was determined by using DPPH free radical scavenging method. The results showed that the tincture has best antioxidant effect at a dose of 100µg/ml when it was compared with ascorbic acid as reference standard.