2017, Vol. 5, Issue 3, Part A

author(s)
Ramdan Btissam, Ramdan Rajae, Greche Hassane, Bakrim Naima and Nhiri Mohamed

abstract
Advanced Glycated End products (AGEs) are involved in the pathogenesis of numerous affections. To improve a protocol for a rapid detection of glycation, in order to screen potential anti-glycation agents, bovine serum albumin (BSA) and methylglyoxal (MG) were incubated at 37 and 50 °C, hourly until 24 h. Electrophoresis and spectrophotometric analysis were used to measure glycated proteins and their aggregations. The anti-AGEs activity screening based on AGEs fluorescence, in 96- well plates, was conducted by incubating BSA and fructose at 50 °C overnight. Quantity of AGEs at 50 °C after 6h of incubation was similar to that at 37 °C after 24h. The screening test showed that four extracts had significant inhibitory effect on BSA glycation (between 40 and 50%). In contrast, some extracts activated this non-enzymatic reaction (between 45 and 60%). The fluorescence determination of BSA (20 mg/ml)-Fructose (0.5 M) system is a rapid and reliable way for anti-glycation phytochemicals screening.