2017, Vol. 5, Issue 3, Part C

author(s)
Victoria Tsygankova, Elena Shysha, Anatoly Galkin, Lyudmila Biliavska, Galina Iutynska, Alla Yemets and Yaroslav Blume

abstract
Impact of microbial biostimulants: Avercom, Avercom nova-2, Violar and Phytovit on induction of callus formation and regeneration of plantlets of wheat (Triticum aestivum L.) of cultivar Zimoyarka on the MS nutrient media supplemented with these biostimulants was studied in vitro conditions. The increase of frequency of formation of callus cells (from 43 up to 74 %) was observed on the apical meristem of 4-5-day-old seedlings of wheat placed on the MS media supplemented with microbial biostimulants: Avercom, Avercom nova-2, Violar and Phytovit used at the concentrations ranging from 10 up to 60 µl/l in combination with 1 mg/l auxin 2.4-D (experiment) as compared with lower frequency of formation of callus cells obtained on the MS media supplemented with only auxin 2.4-D used at the concentration 1 mg/l (control). The increase of efficiency of regeneration of wheat shoots (from 35 up to 65 %) was observed on the 3-4-week-old callus tissue grown on the MS media supplemented with microbial biostimulants: Avercom, Avercom nova-2, Violar and Phytovit used at the concentrations ranging from 20 up to 100 µl/l in combination with 1-2 mg/l cytokinin BAP and 0.5-1 mg/l auxin IAA (experiment) as compared with lower efficiency of regeneration of wheat shoots obtained on the MS media supplemented with 1-2 mg/l cytokinin BAP and 0.5-1 mg/l auxin IAA (control). The rooting of shoots was shown on the MS media supplemented with microbial biostimulants: Avercom, Avercom nova-2, Violar and Phytovit used at the concentrations ranging from 10 up to 100 µl/l. The practical application of microbial biostimulants for increase efficiency of callusogenesis and organogenesis in the isolated tissue culture of wheat was proposed.