Assessment of genetic diversity in Aloe vera L. among different provinces of H.P.
Sabina Rana and Kamlesh Kanwar
The study was undertaken for the assessment of genetic diversity in Aloe vera L. genotypes from different provinces of Himachal Pradesh using morphological, biochemical, Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) markers. On the basis of morphological and biochemical characters very low variation was found among selected genotypes. A total of 30 RAPD and 6 ISSR primers were screened and only 24 RAPD and all the 6 ISSR primers gave amplification. The similarity coefficient value ranged from 0.62 to 0.91 in RAPD. C1 genotype showed its distinct nature at 0.62 similarity index value leaving behind 65-91% genetic similarity among the remaining genotypes. Cluster analysis, using UPGMA, SAHN clustering (NTSYS-pc ver. 2.0) grouped the genotypes into 4 and 2 main clusters in RAPD and ISSR markers respectively. In ISSR markers 57-100% of genetic similarity was obtained, depicting 80% similarity in minor and 65% similarity in major cluster.