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Journal of Medicinal Plants Studies
  • Printed Journal
  • Indexed Journal
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P-ISSN: 2394-0530, E-ISSN: 2320-3862

2017, Vol. 5, Issue 3, Part E

Metabolic profiling and discrimination of Cymbopogon species using direct analysis real time mass spectrometry and principal component analysis

Pratibha Singh, Vikas Bajpai, Sunil Kumar, Mukesh Srivastava and Brijesh Kumar

Several Cymbopogon species such as C. citratus, C. flexuosus, C. nardus and C. khasianus×C. pendulus are extensively used for flavors, fragrance and as folk medicines worldwide due to their volatile essential oils (VEOs). Direct analysis in real time mass spectrometry (DART-MS) method was developed for identification of VEOs from the intact plant parts of ten Cymbopogon species. Total sixteen compounds including VEOs, phenolics and flavonoids were tentatively identified on the basis of their exact mass measurement and molecular formula using DART-MS chemical fingerprint of C. citratus, C. citronella, C. flexuosus, C. pendulus, C. commutatus, Cymbopogon. jwarancusa, C. nardus, C. khasianus, C. jwarancusa× C. nardus and C. khasianus× C. pendulus. DART-MS data was analyzed by principal component analysis and 19 marker peaks were identified which can discriminate among these selected Cymbopogon species. C. citratus, C. jwarancusa, C. commutatus, C. khasianus and C. jwarancusa×C. nardus were approximately overlapping each other while C. flexuosus, C. nardus and C. khasianus×C. pendulus were closer to each other whereas C. pendulus and C. citronella were much apart from all the species. The identified marker peaks could be used for authentication, discrimination and quality control of Cymbopogon species.
Pages : 384-391 | 1547 Views | 183 Downloads
How to cite this article:
Pratibha Singh, Vikas Bajpai, Sunil Kumar, Mukesh Srivastava, Brijesh Kumar. Metabolic profiling and discrimination of Cymbopogon species using direct analysis real time mass spectrometry and principal component analysis. J Med Plants Stud 2017;5(3):384-391.
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