Evaluation of anti-plasmodial, anti-pyretic and mean survival time of rodents treated with ethanolic extract of Enantia chlorantha stem bark
Augustine Ini Lawrence Bassey, Ette Okon Ettebong and Paul Alozie Nwafor
Background and aim of study:Enantia chlorantha is a medicinal plant that is used all over Nigeria for treatment of malaria and other febrile illnesses. Anti-plasmodial effects of the extract and its different fractions (n-hexane, chloroform, ethyl acetate, methanol and aqueous) were tested at doses of 32.40 mg/kg, 64.80 mg/kg and 96.20 mg/kg in Chloroquine sensitive Plasmodium berghei berghei infected mice. The antipyretic effects were evaluated also.
Experimental: Parasitized blood donor of Swiss albino mice with at least 1.0 x 107 parasitized red blood cells was used. Suppressive, prophylactic and curative models were investigated. The mean survival time was observed. Evaluation of anti-plasmodial activity of the fractions using the 4-day test was done. The effect of extract on 2, 4-Dinitrophenol (DNP)-Induced pyrexia in adult albino rats (150-180 g) was also evaluated.
Results: Statistically significant dose-dependent reduction in parasitaemia was noted in all three models (p<0.001) when compared to control. The mean survival times were increased in a dose- dependent manner which was statistically significant (p<0.001). N-hexane, chloroform, ethyl acetate and methanol fractions exerted varying degrees of anti-plasmodial activity with methanol being the highest. The extract treated groups showed dose-dependent reduction in temperature that was statistically significant (p<0.05 – 0.001).
Conclusion: The above results provide support for the traditional use of Enantia chlorantha stem bark in treating febrile illnesses such as malaria in Akwa Ibom state. This extract shows a lot of promise and is a good candidate for evaluation as a potential new drug for malaria and other febrile and inflammatory conditions.
Augustine Ini Lawrence Bassey, Ette Okon Ettebong, Paul Alozie Nwafor. Evaluation of anti-plasmodial, anti-pyretic and mean survival time of rodents treated with ethanolic extract of Enantia chlorantha stem bark. J Med Plants Stud 2017;5(5):16-22.