2017, Vol. 5, Issue 5, Part B

author(s)
Virginia Sarropoulou and Eleni Maloupa

abstract

This study highlights the effect of different micronutrients including copper sulfate (CuSO₄5H₂O) manganese sulphate (MnSO₄H₂O) and zinc sulphate (ZnSO₄7H₂O) combined with 2.22 μM benzyladenine (BA) in MS medium on in vitro shoot proliferation of Sideritis raeseri Boiss & Heldr. The results showed that 0.5-100 μM CuSO₄ increased shoot multiplication from 57.14% (control: 0.1 μM) to 80-100% and 200-1600 μM MnSO₄ from 55.55% (control: 100 μM) to 90-100%. A 2-fold increase in shoot number was recorded with 2.5 or 50 μM CuSO₄ (3 to 5.9-6) or 200 μM MnSO₄ (2.67 to 5.56). Shoot multiplication 100% and 20.98 mm shoot length were achieved with 120 and 240 μM ΖnSO_4, accordingly, compared to the control (30 μM). Therefore, 1-2.5 μM CuSO₄ (x10-25 the normal in MS medium, 0.1 μM), 200μM MnSO₄ (x2, 100 μM) or 240-480 μMΖnSO₄ (x8-16, 30 μM) were proved to be effective for better in vitroshoot regeneration of S. raeseri.