In-vitro skin permeation and biological evaluation of Gingerol and Piperine cream
Rheumatoid Arthritis (RA) is a chronic, inflammatory autoimmune disease causing synovial proliferation and joint destruction. Piperine and Gingerol have been reported to be effective in reduction of Tumor Necrosis Factor-TNF-α, the key inflammatory mediator in RA and have an action on synovial fibroblasts. Essential oils from similar families of oils; such as the florals, herbs, citrus and those that have some similar constituents will normal work together well. Synergy, as used in aromatherapy, is the working together of two essential oils that result in an effect greater than the sum of their individual effects. Thus topical formulation i.e cream containing essential oils containing piperine and gingerol were prepared and evaluated.These formulations showed high retention (60-72%) at the site of action i.e. the dermis, where the fibroblasts are located which was demonstrated by ex-vivo permeation studies. The drug was topically applied to the tibiotarsal joint to evaluate the paw volume was measured up till 28th day, there was less paw edema as measured by plethysmometer.