2017, Vol. 5, Issue 6, Part B

author(s)
Manish Kumar, Binit Kumar, Chanchila Kumari and Namit Kumar Upadhyay

abstract
Aloe vera "cactus," actually a desert succulent, has been touted as one of the miracle plants. It is a complex plant containing numerous biologically active substances. The present study was focused on to minimize a fast and efficient protocol to propagate Aloe vera by tissue culture technique. Murashige and Skoog (MS) basal supplemented with 6-Benzyl Amino Purine (13.5 μM) and α-Napthalene Acetic Acid (8.0 μM) concentration showed best performance as compared to BAP alone (13.3 μM) for bud breaking, and two different combinations of BAP (13.3 μM) + Adenine sulphate (61.8 μM) and BAP (13.5 μM) + NAA (8.0 μM) in MS basal medium showed maximum shoot regeneration and development. The combination of NAA of 10.7 μM with BAP of 8.8 μM recorded the highest percentage (90%) of callus initiation. The best rooting was observed with NAA at 16.1 μM with BAP at 10.7 μM than Indole Acetic Acid (IAA) at 16.1 μM alone.