A potential surface sterilization technique and culture media for the isolation of endophytic bacteria from Acalypha indica and its antibacterial activity
Ramalashmi K, Prasanna Vengatesh K, Magesh K, Sanjana R, Siril Joe S and Ravibalan K
The objective of the current study was to optimize the isolation procedure of the endophytic bacteria from medicinal plant. Medicinal plants are the potential hosts of endophytic microorganisms like bacteria, fungi, actinomycetes etc. Endophytic bacteria live symbiotically within the plant and in turn helping the plant in number of ways like growth, protection to environmental conditions, and sustainability, in favour of the hosts. They produce a wide range of bioactive compounds that are economical importance to humans. The present investigations were undertaken to optimize the isolation and identification of bacterial endophytes in leaf tissue of Acalypha indica plant growing at Coimbatore, India. Studies on the optimization of growth of the isolates were performed by varying growth medium. The ability of bacterial isolates were tested for abtimicrobial activity assay. Future studies will determine the potential medium to isolate endophytic bacteria that can be further applied for various applications like biological control, growth promotion and enzyme production.
Ramalashmi K, Prasanna Vengatesh K, Magesh K, Sanjana R, Siril Joe S, Ravibalan K. A potential surface sterilization technique and culture media for the isolation of endophytic bacteria from Acalypha indica and its antibacterial activity. J Med Plants Stud 2018;6(1):181-184.