Purpose: The aim of this study was to investigate the wound healing activity of ethanolic extract and its toluene fraction of Cucumis melo var momordica Duthie and fuller on excision and incision wound models.
Methodology: The study was conducted on excision and incision wound models. Rats were assigned in to four groups untreated control, 5% povidone iodine ointment, ethanolic extract 200mg/kg body weight and fourth group treated with toluene fraction in a dose of 50mg/kg body weight for both the models. One animal from each group was euthanized on 3rd, 6th, 9th, 14th and 21st days after surgical procedures and the wounded areas were analyzed and removed for estimation of hydroxyproline level, microbial count, tensile strength and total protein concentration.
Findings: Excision wound model exhibits marked increase in wound closure on 3rd, 6th, 9th, 14th and 21st days, dwindled microbial load of wound. Incision wound model shows effective strength in wound breaking strength hydroxyproline level and total protein concentration at the surface of wound site when compared with control animals. Dose dependent changes at (P<0.0001) were found significant.
Conclusion: Based on our findings, we presume that the wound healing potency of toluene fraction was greater on wound contraction, inhibition of microbial count, increased hydroxyproline level, total protein concentration and tensile strength supports the potential for use of toluene fraction of Cucumis melo ethanolic extract as an adjuvant in the treatment of wound healing.
Arvind Kumar Srivastava, Alok Mukerjee, PW Ramteke, Himanshu Pandey, Shanti Bhushan Mishra. Evaluation of wound healing activity of ethanolic extract and its fraction from Cucumis melo var momordica Duthie and Fuller. J Med Plants Stud 2018;6(1):225-228.