Protocol optimization for micro-propagation of Green pepper (Capsicum annum L.)cultivated in Ethiopia
Aragaw Zemene and Abebe Worku
Capsicum annuum L (Green Pepper) is one of the most important types of crop plant cultivated all over the world, and particularly in Ethiopia. A successful protocol of in vitro plant regeneration has been performed using direct shoot bud induction of the explants of this plant. This study was aiming at developing micro-propagation protocol for green pepper genotype growing in Ethiopia. The best sprouting material for our explants by planting it at three different materials like, sand, manure, and mixture of manure and sand has been well assessed. On MS medium containing 6mg/l 6-benzylaminopurine and 1mg/L indole-3-butyric acid has been recorded relatively highest performance for the regeneration of cotyledons. But propagation for the rest of explants like root, shoot tip and hypocotyls was very poor rather all these explants developed into unorganized mass of cells (calli) after allowing them to be cultured for 12 days. Based on the analysis of the study, only in the cotyledons the direct induction that arose around the cut end of the explants has been revealed. It was on MS medium containing 1mg/L BAP+0.5mg/L indole-3-butyric acid, the enlargement of shoot buds was observed. Once they become self efficient, the plants with good root development were allowed to be shifted into the greenhouse conditions where they further developed, more efficient and matured enough with the estimated survival rate of nearly 85%. With this, the study has confidently concluded that the protocol developed can be used for efficient and successful mass propagation of Ethiopian Capsicum annuum L.