Screening of herbal formulation for anticariogenic activity
Shweta N Shouche and Kalpesh B Ishanva
Dental caries and periodontal disease are considered to be most important global oral health burdens. The present study is an attempt to prepare an oral tooth powder herbal formulation effective against microbial flora of oral pathogen. 10 different herbal tooth powder formulations and extracted in four (04) solvent (DMSO, Hexane, Ethyl acetate and Methanol) and its efficacy against four (04) oral (Staphylococcus aureus,Streptococcus pyogenes, Lactobacillus acidophilus and Lactobacillus casei) pathogenicbacteria. Agar well diffusion method and minimum inhibitory concentration were used for this purpose. Among all the solvents, ethyl acetate proved to be the most prominent solvent for extraction of anticariogenic substances from the selected tooth powder herbal formulations. In Staphylococcus aureus, 2 solvents out of 4 have good activity and methanolic extract of formulation no.10 showed highest activity (18 mm). Streptococcus pyogenes, 3 solvents out of 4 have good activity and ethyl acetate extract of formulation no.6 showed highest activity (15 mm). Lactobacillus acidophilus, 2 solvents out of 4 are good activity and methanolic extract of formulation no.5 showed highest activity (22 mm). Lactobacillus casei, 2 solvents out of 4 have good activity and ethyl acetate extract of formulation no.5 showed highest activity (18 mm). Ethyl acetate extracts exhibited moderate MIC values ranging from 0.0156 to 0.5 mg/mL against selected cariogenic bacteria in formulation no.10. Therefore, the herbal tooth powder is effective and has cheaper rate so it is affordable to urban and rural community.