Axillary shoot proliferation from aseptic seedlings of Nepeta nuda Subsp. albiflora
Bengi Erdağ, Yelda Emek, İlknur Kuzu and M Nihan Bağdatl
The objective of the present work was to develop an alternative propagation procedure with in vitro germination and axillary shoot propagation for Nepeta nuda subsp. albiflora (Boiss.) Gams (Lamiaceae) has potential importance as medicinal plant. The seeds were surface sterilized and transferred to different in vitro media. At the end of the experiments, maximum germination percentage was obtained 85% in distilled water medium solidifed with agar. Germinated seeds were transferred onto Murashige and Skoog and White media for further seedling development. MS medium was found to be a superior medium than White medium as seedling growth medium. The obtained shoots were transferred onto MS media containing Benzyladenine, Kinetin and Thiduazuron (0.1, 0.5 and 1 mgL-1) for axillary shoot propagation. MS medium without plant regulator was used as a control. The highest shoot number per explant and maximum shoot lenght were obtained on MS medium supplemented with 0.1 mgL-1Kin.