Antidandruff activity and chemical constituents of the leaves of Betulacylindrostachya Lindl. ex Wall
Chhavi Singla and Mohammed Ali
Dandruff is a skin disorder caused by a fungus Malassezia furfur. There are some medicinal plants and essential oils which possess antidandruff properties. The stem bark and young shoots of Betulacylindrostachys Lindley (Betulaceae) are used as an anti-inflammatory, analgesic, astringent, rubefacient, tonic and to treat bladder infections, neuralgia, rheumatism skin diseases. The air-dried leaf powder was extracted exhaustively with methanol in a Soxhlet apparatus. The concentrated methanolic extract was dissolved in 95% aqueous methanol and fractionated by partitioning the solution with petroleum ether, chloroform, ethyl acetate and n-butanol. The chloroform fraction exhibited marked antidandruff activity against the growth of Malassezia furfur. It was adsorbed on silica gel (60-120 mesh) for the preparation of a slurry. The dried slurry was loaded over silica gel columns. The column was eluted with petroleum ether, chloroform and methanol, successively, in order of increasing polarity to isolate 1-heptadecanol (1), behenyl alcohol (1-docosanol, 2), 1-tricosanol (3), lignoceric acid (n- tetracosanoic acid, 4) and 1-nonacosanol (5). Their structures were established on the basis of spectral data analysis and chemical reactions.