Estimation of nutritive value, total phenolic content and in vitro antioxidant activity of Manihot esculenta Crantz. (Cassava) leaf
Kay Zar Linn and Phyu Phyu Myint
In this research, Manihot esculenta Crantz. (Cassava) sample was collected from Kyon- pyaw Township, Ayeyarwady Region, Myanmar. It has been identified at Botany Department, Hinthada University. In order to find out the types of organic constituents present in the samples, preliminary phytochemical investigation was carried out by test tube and TLC screening methods. The nutritional values such as protein, fiber, fat and carbohydrates were also determined by AOAC method. The total phenolic content of different extracts was determined by Folin-Ciocalteu method. In vitro antioxidant activity was evaluated by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Preliminary phytochemical tests have revealed that the presence of alkaloids, α-amino acid, carbohydrates, glycosides, tannins, phenolic compounds, cyanogenic glycosides, saponins, starch, steroids, terpenoids, flavonoids, and reducing sugar in the sample. Proximate composition of Cassava leaf has been found as 10.70 % of moisture, 3.19 % of crude fat, 29.28 % of protein, 16.25 % of dietary fiber, 33.71 % of carbohydrate, 6.87 % ash and 279 kcal /100 g based on dry sample. The higher extractable total phenolic was recorded in 70 % ethanol extract (748.22 μg GAE/mg) followed by aqueous extract (446.22 μg GAE/mg) for the leaf sample. Both 70 % ethanol and aqueous extracts showed significant DPPH radical scavenging activity. Ethanol extract of (IC50 = 17.69 μg/mL) leaf showed higher level of free radical scavenging activity and found to be the lower in aqueous extract of leaf (IC50 = 42.64 μg/mL). From these results, it can be inferred that this leaf possesses nutrients and antioxidant properties and has nutraceuticals potential for the treatment of malnutrition.