Comparative study of the effect of different extracts of M. Oleifera on some biochemical and histological parameters in rats
Abubakar Amali Muhammad, Dallatu Muhammad Kabir and Asmau Darma Lawal
Moringa oleifera is a plant whose therapeutic significance has been reported scientifically. However, relatively few studies elucidate the safety of different extraction solvents used for the extraction of the plant bio actives. This study investigated the effect of administration of different solvent extracts of M. oleifera on liver and kidney functions of rats. Thirty six adult male rats were used and divided into four groups of nine rats each. Group 1 served as control. Different solvent extracts (aqueous, ethanol, and methanol) were administered to study groups (2, 3 and 4) at different doses of 100, 200 and 400 mg/kg respectively. Biochemical parameters such as total protein, albumin, total and conjugated bilirubin, AST, ALT, urea and creatinine were determined after 3 days (acute) and 28 days (sub-chronic) using standard techniques. Histology of liver and kidney tissues was examined 28 days after administration of the extracts at the highest dose of 400mg/kg. The results showed treatment-related abnormalities in most of the biochemical parameters of methanolic and ethanolic extracts of M. oleifera. However, aqueous extracts did not show any abnormality on the biochemical parameters. Methanolic and ethanolic extracts of M. oleifera significantly increased (p<0.05) the activity of aspartate amino transferase, alanine aminotransferase, total bilirubin, urea and creatinine. It also showed significant decrease (p<0.05) in the levels of total protein and albumin. The significant increase and decreases were however, dose dependent. This was supported by the result of histological evaluation of liver and kidney which did not show any abnormal feature following administration of aqueous extract of M. oleifera. The present study demonstrated some level of safety in the use of aqueous extract of M. oleifera when compared to methanolic and ethanolic extracts that revealed some form of abnormality in the kidney and liver tissues following 28 days of administration. This suggests that, care should be taken in the use of these extracts for their counterproductive consequences resulting possibly from chronic administration of the extract.
Abubakar Amali Muhammad, Dallatu Muhammad Kabir, Asmau Darma Lawal. Comparative study of the effect of different extracts of M. Oleifera on some biochemical and histological parameters in rats. J Med Plants Stud 2018;6(6):84-90.