Solanum torvum Sw. (Solanaceae): Phytochemical screening, antisalmonellal and antioxidant properties of leaves extracts
Flavie Gaelle Djoueudam, Alain Bertrand Fowa, Siméon Pierre Chegaing Fodouop, Norbert Kodjio and Donatien Gatsing
Salmonellosis remains a major public health problem in developing countries, where it remains endemic due to the precariousness of lifestyle combined with the misuse and inappropriate use of antibiotics. In the contination to search for new anti-salmonellosis substances, in vitro antisalmonellal and antioxidant properties of Solanum torvum leaves extracts were evaluated.
The Minimal Inhibitary and Minmal Bactericidal Concentrations (MBCs) were determine using broth dilution method. The in vitro antioxidant activity of thes extracts were alsomine using DPPH and Nitric oxide tests, Hydroxyl radicals scavenging assaysand iron reduction. Total phenol as well as flavonoid contents and phytochemical screening were performed by standard methods.
In vitro antimicrobial test results showed that the activity of the 95° ethanol extract were significant (MIC <100 μg/mL) on all bacterial isolates and strain. Follow by ethanol extract 70% whose activity was significant on 4/5 of the bacteria tested. The antioxidant test results showed that 95° and 70% ethanol extracts have a high antioxidant potential (IC50 < 20 μg/mL) on DPPH. This extract (95° ethanol) showed the best percentages of nitric oxide (62.43%) and hydroxyl radical (49.97%) trapping and has a good ability to chelate iron. These activities correlate significantly with the levels of phenolic compounds and flavonoids. The greater the amount of these compounds, the greater were the radical scavenging effect. The phytochemical test of extracts of S. torvum revealed the presence of anthocyanins, saponins, steroids, tannins, anthraquinone, alkaloids, flavonoids, triterpenes, phenols.
These results show that the 95° ethanol extract of Solanum torvum has interesting antisalmonellal and antioxidant activities in vitro and could be use for the formulation of antisalmonellal phytomedicines.