Antityphoid and antioxidant activities of hydroethanolic leaf extract of Adenia lobata Jacq. (Passifloraceae) on Salmonella typhi infected wistar rats
Alain Bertrand Fowa, Siméon Pierre Chegaing Fodouop, Charles Ntungwen Fokunang, Flavie Gaelle Djoueudam, Louis-Claire Ndel Famen, Nadine Sylvie Ongbayokolak and Donatien Gatsing
Typhoid fevers are a major public health problem, especially in developing countries where they are endemic. In order to contribute to the search for new antityphic substances, medicinal plants are a good source of new antityphic molecules accessible by everyone. In this study, the in vivo antisalmonellal activity of the hydroethanolic leaves extract of Adenia lobata was evaluated in albino rats infected with Salmonella typhi (ATCC 6539) and the antioxidant activity of this extract was evaluated after treatment.
In vivo antisalmonellal activity was assessed by the blood culture method on Wistar rats infected by S. Typhi (ATCC 6539), and oxidative stress biochemical parameters (superoxide dismutase, catalase, glutathione, malondialdehyde, nitric oxide) were evaluated after treatment. Phytochemical screening was performed to justify these activities.
The hydroethanolic leaves extract of Adenia lobata at different doses (5. 45 and 90 mg/kg of body weight) resulted in the healing of infected rats between the eighth and twelfth day of treatment. Extract treatment reduced malondialdehyde levels and increased SOD, catalase, glutathione, and nitric oxide levels in infected rats by normalizing some of this content compared to neutral controls. Phytochemical analysis revealed the presence of flavonoids, alkaloids, steroids, tannins, phenols, saponins, anthocyanins, triterpenes and anthraquinones in the hydroeyhanolic leaves extract of Adenia lobata.
These results show that the hydroeyhanolic leaves extract of Adenia lobata cures typhoid fever and reduces the state of oxidative stress caused by S. typhi during infection.