Detection of phytochemicals and in vitro propagation of Banana (Musa variety Gaja Bantal)
Bikram Pradhan and Bandita Deo
The present experiments were conducted to evaluate the phytochemicals present in the sap and stem samples of a Musa variety, Gaja Bantal. Attempts has also taken to study various effects of plant hormones such as cytokinin (BAP) and auxins (IAA and NAA) on shoot proliferation and root induction during its in vitro micropropagation. Results from various experiments showed that the sap as well as stem extraction contain high phenolic and flavonoid content. Due to heavy phenolic content lethal browning of explants was observed during different stages of in vitro propagation Gaja Bantal. While both the samples (Sap and Stem) contain alkaloids, Saponin was slightly detected only in the stem sample. During micropropagation of Gaja Bantal it was observed that media containing high concentration of BAP (8 mg/l) had better respond in initial culture while low concentration of BAP (3 mg/l) had high bud formation in multiplication stage. Gaja Bantal explants grown on multiplication medium supplied with 3 mg/l BAP + 0.5 mg/l IAA had highest numbers of shoot buds. In rooting culture the media containing 1 mg/l IAA as well as media containing 1 mg/l NAA found to be more effective in root induction.