Comparative GC-MS analysis of two crude extracts from Eremomastax speciosa (Acanthaceae) leaves
Gaël Tchokomeni Siwe, Nkwengoua Zondegoumba Ernestine, Rukesh Maharjan, André Perfusion Amang, Christophe Mezui, Muhammad Iqbal Choudhary and Paul Vernyuy Tan
This study was designed to identify the possible volatile compounds present in two extracts from Eremomastax speciosa leaves. The air-dried leaves were powdered and subjected to extraction using respective solvents; distilled water and methanol/methylene chloride. Then, each of the extracts was further subjected to gas chromatography–mass spectrometry (GC-MS). GC-MS analysis revealed the presence of 22 compounds in aqueous extract and 44 compounds in methanol/methylene chloride extract. Five compounds were found commonly in both extracts. The major constituents identified in aqueous extract were olean-12-en-3-one (28.37%), 2, 7-Dioxaisotwistane (19.77%), 9-oxabicyclo [3.3.1] nonane-2, 6-diol (15.80%) and α-Amyrin (12.09%). In methanol/methylene chloride extract the major compounds identified were n-hexadecenoic acid (17.74%), stigmasterol (12.81%), γ-sitosterol (10.50%) and α-Linolenic acid (9.01%). Some of these compounds were already reported to be pharmacologically active. From these results and given that Eremomastax speciosa is widely used to threat or prevent many diseases, it is obvious that this plant contains many biologically active compounds and might be exploited for the development of plant-based drugs.