The pharmacognostic and antimicrobial study of methanolic extract of Dialium guineense leaf against organisms isolated from wound infection
Fidelia Ijeoma Uche, Dickson Onuchukwu and Hanson Ige Ogbu
There is a growing interest worldwide in the use of natural product of various plants as a natural antimicrobial agent especially now where the problems of emerging and reemerging resistant strains of microorganism are becoming the order of the day. The phytochemical screening and antimicrobial activities of methanolic extract of Dialium guineense leaf was tested to provide a basis for their adoption as an alternative control measure in combating this incidence. The Pharmacognostic/phytochemical screening were carried out using standard methods. Antimicrobial activity was tested against clinical isolates of Proteus mirabilis, Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Aspergillus fumigatus and Candida albicans using agar well diffusion method. Results of the Pharmacognostic studies revealed the presence of anticlinal walls which are thick and straight, numerous Uniseriate covering trichomes in both upper and lower epidermis. The phytochemical analysis showed presence of carbohydrate, tannins and alkaloids. The antimicrobial inhibition zone values of the extract ranged between 4 and 18 mm, with E. coli being most sensitive, followed by A. fumigatus and P. mirabilis with least effect. The finding suggests that D. guineense might be a good candidate in the search for a natural antimicrobial agent and further supports its popular and wide traditional applications in the treatment of various illnesses. Further study is ongoing to identify the specific secondary metabolites present in D. guineense which could be responsible for its antimicrobial activity. Study also needs to be done to determine the toxicological profile of the active principles in this plant.
Fidelia Ijeoma Uche, Dickson Onuchukwu, Hanson Ige Ogbu. The pharmacognostic and antimicrobial study of methanolic extract of Dialium guineense leaf against organisms isolated from wound infection. J Med Plants Stud 2019;7(2):39-44.