2019, Vol. 7, Issue 4, Part C

author(s)
Dhananjay Desai, Vishal Dhundale, Kavita Kasar and Dhanashree Desai

abstract
Objective: To study antibacterial activity of various extracts of leaves and bark of G. indica (Kokum) against E. coli, Klebsiella spp. and Salmonella spp. and their qualitative phytochemical investigation. Materials and Methods: Six different solvent viz. chloroform, hexane, methanol, ethyl acetate, acetone and aqueous were used for extract preparation. Total 12 extracts were prepared form bark leaves and bark of G. indica. Agar cup diffusion method was used to evaluate qualitative antibacterial activity against E. coli, Klebsiella spp. and Salmonella spp. qualitative phytochemical presence also evaluated by previously described standard protocols with suitable modifications. Results: All extracts showed considerable antibacterial activity against tested bacterial spp. Hexane, aqueous and acetone extract of leaves was found more antibacterial activity against E. coli, Klebsiella spp. and Salmonella spp. respectively. Extracts prepared from bark of G. indica also showed moderate antibacterial activity against same tested bacterial spp. In that case methanol, chloroform and acetone extracts showed potential antibacterial activity against E. coli, Klebsiella spp. and Salmonella spp. respectively. Acetone extract of leaves and bark are almost equally effective against salmonella spp. it is might be due to the presence of phytochemicals in it. Phytochemicals screening of the both acetone extract showed positive for carbohydrate, flavonoid and tannins in both the acetone extracts, but in both extract acetone extract of bark showed maximum appearance of phytochemicals than the leaves. Conclusions: The results obtained from present study showed that the G. indica is a potential source of various phytochemicals and it can be used in the treatment and prevention of against bacterial infections.