Poly phenolic constituents of Dalbergia paniculata roots
Dr. Meer Altaf Ahmed and Dr. MVNV Prasad Gupta
Flavonoids are biologically active polyphenolic compounds widely distributed in plants, are biogenetically derived from cinnamoyl-CoA and three molecules of malonyl-CoA, and contain a C6+ C3+C6 structural unit. On the basis of an in vitro primary screening test many kinds of flavonoids and isoflavonoids, including flavones, flavanones, flavanols, flavanonols, isoflavones, chalcones, and catechins, showed inhibitory effects on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced Epstein-Barr virus early antigen (EBV-EA) activation. The stem bark of Dalbergia cultrate Grah. And D. nigrescens Kurz (Leguminosae) were found to exhibit a significant sources of flavonoids are vegetables, fruits, nuts, seeds, roots, and beverages like tea and wine. Health effects of tea have been recognized since the ancient times. In a tea polyphenols could play a role in the prevention of cancer and heart disease. To date, the most protective effect is shown against lung cancer. The major antioxidant activity of tea extracts is attributed to Epigallocatechin gallate. Besides the catechin molecule, which is a potent antioxidant, gallate moiety is an important contributor to the antioxidant activity.
Plant belonging to Dalbergia genus exhibit different physiological activity, such as general tonic and as cure for blood troubles, inflammation, ulcers, tumors, and leprosy and also employed as a in insect repellent and is infectant. The flowers and bark of Dalberigia paniculata (Roxb) is widely distributed throughout Southern and Central India. Its vernacular name is “patsaru”.
The roots air dried (1Kg) was powdered in a pulveriser and extracted dichloromethane and methanol (1:1) (3lit) in a room temperature for 5 days for each time and 24h soxhlet extractor. The combined extracts were concentrated separately under vacuum to yield gummy semi solids (125gm). This extract was suspended in water and then was further extracted with EtOAc and n-Butanol to yield soluble faction of EtOAc and n-Butanol (45gm). This fraction was subjected to a chromatography (CC) over silica gel eluted with different solvents of increasing polarity (hexane: acetone and DCM: Methanol from 10:1 to 1:1).